Description
Detect active serine protease enzymes with the FAM-Leu-CMK Green FLISP Assay. This in vitro assay employs the green fluorescent label carboxyfluorescein (FAM), which is linked to the target amino acid leucine, and a chloromethyl ketone (CMK) reactive group, forming the fluorescent inhibitor probe, FAM-Leu-CMK. FAM-FLISP labels active chymotrypsin-like serine proteases in living cells. Analyze samples using fluorescence microscopy, fluorescence plate reader, or flow cytometry.