The MitoPT® TMRE Assay detects mitochondrial membrane depolarization utilizing the fluorescent dye TMRE. When accumulated in a negatively charge polarized mitochondria, TMRE fluoresces orange. When mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, TMRE reagent is dispersed through the cell cytosol and fluorescence levels drop dramatically. Analyze your results using a flow cytometer, fluorescence plate reader, or fluorescence microscopy.
MitoPT® is a trade mark of Immunochemistry Technologies, LLC