Description
This inhibitor reagent employs an O-phenoxy (OPH) reactive group instead of an FMK group. In a manner analogous to the FMK class of cysteine reactive compounds, OPH inhibitors form a stable covalent thioether adduct with the reactive SH-site of caspase enzymes present in apoptotic cells. These reagents are used as simple and reliable methods for screening apoptosis in live cells in tissues. FAM-VAD-OPH I (5-FAM-Val-Ala-Asp(OMe)-2,6-difluorophenoxy-methylketone poly caspase inhibitor) can be used with a fluorescence microscope, fluorescent plate reader, or flow cytometry.