Description
Total Cytotoxicity & Apoptosis Assay is a single-tube, tri-color assay for quantitative assessment of cell-mediated cytolytic activity due to apoptosis and necrosis. The assay employs a green fluorescent cellular stain, CFSE, to label target cells, the red live/dead viability dye, 7-AAD, to identify the dead cells present in the cytotoxicity assay samples, and the orange-red SR-FLICA reagent, SR-VAD-FMK, to measure caspase activity in the target cell population. Analyze your results using a flow cytometer.