News

Sertraline is a selective serotonin reuptake inhibitor that is well-known for its use in treating various psychiatric disorders. It is certainly involved in serotonin regulation. However, its impact may also extend beyond that and into the expression of genes tied to neurobiology. Recent studies have also investigated the role of sertraline in other pathologies including cancer pathways and neurochemistry stress response.

In a new study at the National Autonomous University of Mexico, the impact of sertraline on fruit flies was investigated. The fruit fly larvae were submitted to a single exposure of sertraline during their third instar larval stage. Amazingly, the effects of a single 24 hour exposure in early-life stage caused notable changes that were observable in the grown adult flies. These changed included significant effects on alternative splicing of genes that deal with neuronal function and structure.

The most remarkable finding of this study was the lasting impact of the single pharmacological exposure early in life. The single exposure was sufficient to modulate gene expression through the lifetime of the fly. In this case, the impact of sertraline seemed to be positive. It enhanced the autophagy machinery, resulting in a persistent neuroprotective effect.

Further studies using vertebrate models are needed to further assess the full potential of sertraline to modulate key genes involved in neuronal function.

Santos-Cruz L, Campos-Aguilar M, Castañeda-Partida L, et al. Impact of larval sertraline exposure on alternative splicing in neural tissue of adult Drosophila melanogaster. Int J Mol Sci. 2025 Jan 10;26(2):563. PMID: 39859278

Acepromazine is a derivative of chlorpromazine which is useful as a veterinary research tool. It has been shown to decrease packed cell volume, a.k.a. hematocrit, a measurement of the proportion of blood that is made up of cells.

Coagulopathy is a common issue in equine illness and requires proper attention and management. Recognizing, predicting, and treating abnormal coagulation is essential. Recently, an equine study made use of acepromazine as a research tool in a study of coagulopathy.

The study involved eight healthy horses and intravenous administration of acepromazine to induce anemia. Several viscoelastic parameters were then evaluated in order to establish the relationship between blood cell mass, hypercoagulability, and hypocoagulability. Understanding these relationship dynamics is essential to correctly interpreting and test results that are produced by an anemic subject.

The results showed acepromazine to be successful in inducing anemia in this model, and showed a connection between red blood cell mass and hypercoagulability. Although this study was limited in size, it does confirm the very different testing profiles found in anemic versus polycythemic subjects. Further studies could increase the understanding and develop appropriate reference ranges expected under these conditions.

Mersich I, Bishop R, Yucupicio S, et al. Decreased circulating red blood cell mass induced by intravenous acepromazine administration alters viscoelastic and traditional plasma coagulation testing results in healthy horses. Animals (Basel). 2024 Oct 28;14(21):3102. PMID: 39518825

 

Hydroxytyrosol is a naturally occurring polyphenol with remarkable antioxidant activity. Its precursor oleuropein is present in olive fruits but also in all parts of the olive tree. While oleuropein has several known biological activities, the activity of hydroxytyrosol may be even greater. Therefore, obtaining as much as possible hydroxytyrosol, rather than oleuropein, from the plant is desirable.

The agricultural waste produced from annual pruning of olive trees is normally disposed of by burning. However, these leaves and small branches also contain oleuropein. Here there is an opportunity to extract a high-value product from what is generally thought to be a waste material.

Recently, researchers have attempted to develop and optimize just such a process. Enzymatic reactions were developed to convert the original oleuropein into the desired hydroxytyrosol. Enzymes with varied characteristics such as molecular size, isoelectric point, and hydrophobic domains, were tested.

With a two-step reaction strategy, first the glucosidic bond was broken, followed by hydrolysis of the aglycon. They found that the first step alone required 7 days, at low concentration, and the second step did not work as expected, but seemed to also require enzyme action. The outcome was only 5% transformation.

They also examined a one-step reaction scheme using a different type of enzyme, and this process allowed for a higher concentration and obtained 30% transformation in 12 days. This process was much more effective than the two-step strategy.

However, with only 30% transformation, it may be possible to further optimize the process to obtain even better yield of hydroxytyrosol. On the whole, using a waste material to create a valuable product is bound to be a worthwhile endeaver.

Garcia-Molina G, Peters E, Palmeri R, et al. Enzymatic synthesis of hydroxytyrosol from oleuropein for valorization of an agricultural waste. Bioengineered. 2024 Dec;15(1):2396647. PMID: 39235136

Betulinic acid is a natural product found in several plant sources including white birch bark. Interestingly, this molecule has several biological activities, notably antioxidant properties. Studies indicate that the antioxidant activity of this molecule is mediated through the Nrf2/Keap1 signaling pathway, which defends against oxidative stress.

Oxidative stress is one of the major causes of in vitro oocyte maturation defects. Therefore, optimizing the in vitro conditions will lead to more successful outcomes. Undeniably, this is an important step in the process of assisted reproductive technology.

Recently, a study looked at the impact of betulinic acid on the maturation of porcine oocytes. First, the cumulus-oocyte complexes (COCs) were extracted from porcine ovaries. The the COCs were allowed to mature in vitro in the presence or absence of various concentrations of betulinic acid. Next, the matured oocytes were parthenogenetically activated, followed by incubation for 6 days.

As it turns out, the COCs treated with betulinic acid showed enhanced meiotic progression leading to more successful outcomes. Anaylsis indicates that the treatment regulated antioxidant genes, thereby reducing the intracellular oxidative stress level.

Now that these positive in vitro results have been observed, it will be necessary to further evaluate for any long-term effects on the embryonic development. This research is important for the improvement of in vitro maturation systems.

Additionally, related compounds are also available from LKT Labs:

Betulin

Betulin-3-Acetate

Kim MJ, Kang HG, Jeon SB, et al. The antioxidant betulinic acid enhances porcine oocyte maturation through Nrf2/Keap1 signaling pathway modulation. PLoS One. 2024 Oct 10;19(10):e0311819. PMID: 39388445

 

Benfotiamine is a synthetic derivative of the B vitamin thiamine. There are 8 types of vitamin B ! This derivative contains an open thiazole ring allowing it to cross the blood-brain barrier. The ability to cross the blood-brain barrier gives it significantly increased bioavailability. This may prove to be useful in controlling diseases involving thiamine deficiency, such as Parkinson’s disease.

Thiamine and Dopamine

Parkinson’s disease is noted to have decreased levels of dopamine in the brain. Thiamine is involved in the synthesis and release of dopamine, an important relationship to note. Studies have shown that the level of free thiamine found in cerebrospinal fluid is lowered in Parkinson’s patients compared to healthy patients. Therefore, it may be possible that adding thiamine may result in a normal level of dopamine synthesis and decelerate or treat Parkinson’s.

Experimental Modelling

Recently, a study using a mouse model of the disease explored that possibility. The toxin MPTP is known to selectively damage dopaminergic neurons in the substantia nigra. This tool is often used to replicate the characteristics of Parkinson’s disease. In the study, the mice were treated with MPTP by intraperitoneal  injections for 5 days to develop the disease characteristics. Some of the groups were also given benfotiamine by oral administration for 28 days.

Testing and Results

Next, the mice were subjected to behavioral tests including the pole test, hang test, step distance experiment, and open field test. The mice that received treatment of benfotiamine showed improved performance of several motor functions.

Finally, brain tissues were collected and analyzed. These results showed that the treatment increased the number of dopaminergic neurons and partially replenished dopamine and its metabolites. Overall, benfotiamine was credited with the neuroprotective effect.

This study gave some very positive results that should be further investigated. Additional models of Parkinson’s disease are available and should also be used. Testing with an increased sample size and possibly some forms of combination therapies should also be considered.

Wang K, Han C, Yang J, et al. Benfotiamine protects MPTP-induced Parkinson’s disease mouse model via activating Nrf2 signaling pathway. PLoS One. 2024 Jul 23;19(7):e0307012. PMID: 39042624

Ginsenosides are the naturally occurring steroid compounds found in ginseng. Approximately 100 different ginsenosides have been identified so far and most of these have some biological activity including anti-tumor effects.

Recently, the effects of ginsenoside Rg1 against the malignant tumors of multiple myeloma were observed. Multiple myeloma is generally treated with bortezomib, however, the emergence of drug resistance against this treatment has increased in recent years. There is need for a new treatment or combination treatment to battle this cancer.

In a new study at Ningbo University, a drug-resistant cell line RMPI8226R was constructed. Next, a four-week-old nude mouse model was subcutaneously inoculated with either the ‘normal’ cancer cells or these drug-resistant cancer cells. All of the mice developed tumors, which were then allowed to grow to a predetermined size. Once the tumors were well-developed, treatments were begun.

The treatments involved intraperitoneal injections of either bortezomib, or ginsenoside Rg1, or a combination of bortezomib + ginsenoside Rg1. Injections were continued every 3 days for 15 days. After that time, the tumors were collected and analyzed.

The results showed that treatment with ginsenoside Rg1 was able to inhibit the development of multiple myeloma tumors. It was also found that the addition of ginsenoside Rg1 to bortezomib treatment was able to overcome the drug resistance. This proved the anti-tumor effect of ginsenoside Rg1, however, only one multiple myeloma cell line RMPI8226 was investigated so far. Further research including additional cell lines is needed.

Ginsenoside Rg1 as well as several other ginsenosides are available from LKT Labs: see all

Lin L, Chen D, Li S, et al. Ginsenoside Rg1 inhibits multiple myeloma and overcomes bortezomib resistance through AMPK-mTOR pathway. Heliyon. 2024 Jul 1;10(13):e33935. PMID: 39071579

PF-07321332 is an active ingredient in Paxlovid, which is a treatment for COVID-19. This molecule inhibits the activity of the main protease of the virus SARS-CoV-2. This main protease is responsible for producing 12 functional proteins which are highly conserved across several coronaviruses. This includes several of the well-known COVID-19 variants.

Finding a treatment that can remain effective against various mutant varieties as well as the wild-type is key to combating this virus. Recently, the D48N mutant was investigated in comparison to the wild-type for effectiveness of the treatment. The D48N mutant contains a single amino acid substitution near the main protease active site. This substitution has already been confirmed to exist in several COVID-19 variants.

To begin the study, first the D48N mutant was cultured and purified. The next step was to run enzymatic inhibition assays to test the inhibitory activities of both shikonin and PF-07321332. Shikonin was also included in the test because the research team’s previous work shows that it also appears to be promising for main protease inhibition.

Next, the purified D48N was mixed together with either of these molecules and allowed time to bond, resulting in the formation of a complex. The complex was then crystallized and the crystals collected for examination. By this method the structures of the complexes can be determined and the interaction of the molecule with D48N confirmed.

The results showed that both PF-07321332 and shikonin have potent inhibitory effects against both D48N mutant and wild-type main protease. Interestingly, shikonin demonstrated non-covalent binding while PF-07321332 demonstrated covalent binding. The data from this study may be useful in further work to develop additional treatments for COVID-19.

Zhao Z, Zhu Q, Zhou X, et al. Structural basis for the inhibition of SARS-CoV-2 Mpro D48N mutant by shikonin and PF-07321332. Viruses. 2023 Dec 30;16(1):65. PMID: 38257765

Levosimendan is a calcium sensitizer that produces a potent effect on the heart muscle. Acting as a positive inotrope, it strengthens the heart’s contractions, making it pump more efficiently. In comparison with other inotropes, levosimendan does not cause unwanted arrythmogenic effects.

In an aging population, chronic diseases are expected to have increasing prevalence. One such disease is heart failure with preserved ejection fraction (HFpEF).

Recently, an animal study was completed to investigate the possibility that HFpEF may be controlled using this inodilator. ZSF1 obese rats were used as a preclinical surrogate model for HFpEF. The animals were treated for 6 weeks with 1 mg/kg/day levosimendan in drinking water. Another group was submitted to i.v. levosimendan infusion to determine the acute effects.

Both types of treatment seemed to show beneficial haemodynamic effects in this study. However, as with many animal models of human diseases, this model has some limitations. Further research should carefully appraise the effectiveness of this treatment on an aging population of HFpEF.

Moreira-Costa L, Tavares-Silva M, Almeida-Coelho J, et al. Acute and chronic effects of levosimendan in the ZSF1 obese rat model of heart failure with preserved ejection fraction. Eur J Pharmacol. 2024 Mar 5:966:176336. PMID: 38272343

Caerulomycin A is a microbial metabolite isolated from marine actinomycetes. The chemical structure consists of a 2,2′-bypyridine core, which is similar to a biphenyl core. Because of this similarity, it is thought that it may bind well to tubulins and DNA topoisomerase, and thereby provide a potent anti-cancer affect.

In vitro and in vivo studies have shown caerulomycin A to be effective against paclitaxel-resistant cancer. In a study at Ohio University, several types of human tumor cell lines were prepared in vitro and treated with caerulomycin A and in combination with paclitaxel. The melanoma cells were also injected subcutaneously into a mouse model for in vivo study. The mice were then administered caerulomycin A by intraperitoneal injections.

The in vitro studies showed the growth of all cancer types was inhibited, including the paclitaxel-resistant cancer cells. The animal model was also effective and confirmed a significant reduction in tumor size.

The results of this study suggest a synergistic effect when used in combination with paclitaxel against several cancer types. Further work with caerulomycin A and related compounds may offer a new strategy for cancer treatment.

Tong L, Sun W, Wu S, et al. Characterization of caerulomycin A as a dual-targeting anticancer agent. Eur J Pharmacol. 2022 May 5:922:174914. PMID: 35337812

 

Virginiamycin is commonly used in livestock as a growth promoter. In the final stage of cattle farming, the dietary components of the feedlot are adjusted to produce the best quality meat. This generally involves increasing the amount of fat in the animal. In most cases, the final stage diet is grain-based, rather than grass. During this stage, additives such as virginiamycin are also introduced.

Virginiamycin is a macrolide antibiotic which has repeatedly been shown to improve animal performance. However, it is unclear whether it works by protecting the liver, or impacting the short-chain fatty acid profile in the rumen, or improving the protein flow to the small intestine.

Recently, a study at Texas A&M evaluated the effects of this additive in an attempt to understand exactly how it works. The animals were treated with 3 different dosage levels for comparison. After treatment, samples of rumen fluid, feces, and urine were collected and analyzed.

The results from this study found that virginiamycin supplementation did not effect nutrient digestibility but did increase ruminal pH and impacted the rumen dynamics. It also seemed to improve the protein flow to the small intestine.

Additional research should be done to further understand the impact of virginiamycin supplementation on the bacteria population and fermentation in the rumen. Laboratory reference standards for specific virginiamycins are available at LKT Labs:

Virginiamycin M1

Virginiamycin S1

Virginiamycin Mixture (M1 & S1)

Batista L, Rivera M, Fonseca M, et al. The influence of virginiamycin on digestion and ruminal parameters under feedlot conditions. Transl Anim Sci. 2024 Feb 14:8:txae019. PMID: 38406320

Capsaicin has been identified as the primary component that contributes to the hotness of chili peppers. Around the world, chili peppers are a popular ingredient used in many kinds of food. There are many varieties of chili pepper and each offers a slightly different flavor and level of heat. In some communities they are even used as a food staple.

Over time, this ingredient and its possible role in cancer has become a subject of controversy. While some studies with capsaicin have shown that it can inhibit cancer cell growth and metastasis, other studies have shown the opposite result.

Recently, a study at Nanjing University focused specifically on a diet with high level of capsaicin and its impact on gastric cancer. This study used a xenograft mouse model of gastric cancer fed with a diet of either 50 mg/kg or 100 mg/kg capsaicin. This is the equivalent of a human consuming 90-250 mg capsaicin each day. The results showed that this type of diet led to increased metastasis rates and increased number of cancer nodules. Interestingly, the size of the tumors did not increase.

While other experiments have given evidence of capsaicin having anticancer properties, this study gives evidence of its cancer promoting properties. One key difference between studies is likely to be the concentration or intensity of the application. Perhaps at lower concentration and frequency of consumption, there is a beneficial effect, but with continual high concentration intake as in this study, the effects become detrimental. It seems chili peppers provide yet another example of ‘moderation is key’.

Deng R, Yu S, Ruan X, et al. Capsaicin orchestrates metastasis in gastric cancer via modulating expression of TRPV1 channels and driving gut microbiota disorder. Cell Commun Signal. 2023 Dec 21;21(1):364. PMID: 38129926

Acetylcholine neurotransmission is thought to be important in combating Alzheimer’s disease. When acetylcholine transmission is reduced, cognitive impairment as seen in Alzheimer’s patients is observed. Therefore, maintaining or increasing the level of acetylcholine in the brain is the strategy to treat this disease.

There are two known enzymes that catalytically hydrolyze acetylcholine in the brain. These are acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). AChE is the predominant enzyme, while BChE is thought to have a supportive role. Because of that, AChE inhibition has traditionally been the goal to set. However, as the disease progresses, it is noted that AChE levels progressively decrease while BChE levels increase. Eventually BChE becomes the more abundant enzyme. As such, at later stages of the disease, the more effective battle may be against BChE.

Another strategy is to use a molecule that acts as inhibitor for both AChE and BChE. Rivastigmine is such a dual inhibitor and is currently used in treatment of dementia. However, rivastigmine is more selective for AChE than BChE. Another molecule, bambuterol, is more selective for BChE than AChE. Therefore, a hybrid analogue molecule of rivastigmine and bambuterol may have improved potency and selectivity than either molecule alone.

To test this idea, 15 hybrids were synthesized and evaluated in vitro for their capability as AChE and BChE inhibitors. As it turns out, each of these hybrids is a stronger inhibitor of BChE than bambuterol. One of them also had longer-lasting inhibition. These hybrids are a good starting point in searching for new candidates for Alzheimer’s research.

Wu J, Tan Z, Pistolozzi M, et al. Rivastigmine-bambuterol hybrids as selective butyrylcholinesterase inhibitors. Molecules. 2023 Dec 22;29(1). doi: 10.3390/molecules29010072. PMID: 38202655

 

Inflammatory bowel disease is a group of inflammatory conditions that usually occurs as either Crohn’s disease or ulcerative colitis. In Crohn’s disease, any part of the digestive tract may be involved, most commonly the small or large intestine. In contrast, ulcerative colitis involves only the large intestine. The exact causes of these diseases are unknown. One thing that is clear is that, over time, the gut microbiome is disrupted.

A New Strategy:

One possible way to combat these diseases may be to boost the microbiome back to its normal function. A recent study was performed to attempt just that. Researchers used a mouse model of immune-modulated Crohn’s disease. The mice were fed a diet including raw broccoli sprouts, which contains the anti-inflammatory compound sulforaphane. Sulforaphane in its purified chemical form has previously been shown to protect mice and their gut from ulcerative colitis.

After 7 days, the mice were inoculated with Heliobacter hepaticus, which induced the symptoms of Crohn’s disease. The broccoli sprout diet was then continued for another 2 weeks.

Evaluation:

A healthy gut microbiome must consist of a large diversity of families in the bacterial community. The populations of the good bacteria must be able to thrive over common gut pathogens. The gut microbiome has a changing microbial community early in life and begins to stabilize around adolescence. Therefore, the study included 2 sets of mice of different ages, one group was very young and at post-weaning, the other group was at adolescence. The dietary intervention was found to be much more beneficial for the very young group.

Treatment for inflammatory bowel disease requires consideration of so very many factors. Further research will be needed to confirm whether or not the benefits to the very young group would carry on into adulthood. If so, this will be a good step in learning how to manipulate the gut microbiome to ensure continued good health.

Several forms of Sulforaphane are available at LKT Labs:

R,S-Sulforaphane, Research Grade (≥97%)

R,S-Sulforaphane, High Purity (≥99%)

R-Sulforaphane, Research Grade (≥97%)

R-Sulforaphane, High Purity (≥98%)

S-Sulforaphane (97%)

Holcomb L, Holman J, Hurd M, et al. Early life exposure to broccoli sprouts confers stronger protection against enterocolitis development in an immunological mouse model of inflammatory bowel disease. mSystems. 2023 Dec 21;8(6):e0068823. doi: 10.1128/msystems.00688-23. PMID: 37942948

 

 

Gastric cancer has severe morbidity and mortality rates and is the fifth most common cancer globally. This cancer is known to metastasize aggressively, making it very challenging to control. Research has shown histone deacetylase 6 (HDAC6) being related to this specific cancer and has therefore become a target in searching for an effective treatment.

Carbenoxolone disodium has been identified through the use of high-throughput screening as an inhibitor of HDAC6. Carbenoxolone disodium is an active metabolite of glycyrrhizic acid, a natural compound known for its anti-inflammatory properties. A recent study at Zhengzhou University tested the effectiveness of carbenoxolone in cell culture studies and in a mouse model.

In the cell culture studies, normal gastric cells were tested alongside two types of gastric cancer cells. The test results showed that carbenoxolone could block the growth of the cancer cells without showing toxicity to the normal cells. This indicates that this chemical may be a relatively safe agent. The tests also showed that carbenoxolone blocked the wound healing of the cancer cells, inhibiting the migration capacity. And finally, the process of epithelial-mesenchymal transition was reversed after the treatment. Epithelial-mesenchymal transition is an important process allowing cancer cells to increase their mobility.

In the animal study, nude mice and the gastric cancer cells were used to establish a lung cancer metastasis model. Afterwards, oral treatment with carbenoxolone was administered. The results of this experiment showed that carbenoxolone treatment significantly inhibited the lung metastasis.

This study confirms carbenoxolone disodium to be a selective and potent inhibitor for HDAC6 in gastric cancer cells. This is a good lead for development of an anti-metastasis drug against gastric cancer.

Song Z, Zhao C, Yan J, et al. Carbenoxolone disodium suppresses the migration of gastric cancer by targeting HDAC6. Future Med Chem. 2023 Feb;15(4):333-344. doi: 10.4155/fmc-2022-0246. PMID: 36946221

 

Pharmaceuticals are one of the main groups of organic contaminants found in wastewater. As they are repeatedly pushed into the environment and returned to the water supply, even at low concentrations, a negative impact begins to build. These impacts are felt by humans and animals, and the chemicals can be absorbed by crops, causing further problems.

The Design Problem:

Most wastewater treatment plants are designed to remove suspended solids. They are generally not able to remove pharmaceuticals. The widespread and ever-increasing use of pharmaceuticals only increases the amounts of these materials being excreted into the wastewater. Therefore, new treatment methods that can remove these materials must be developed.

Researchers at the University of Granada are now searching for a solution. While traditionally the wastewater treatment plants use an activated sludge technology, alternative new technologies are being designed using membrane bioreactors. The membrane bioreactor provides improved process control and treatment effectiveness. However, the effects of some of the more frequently detected pharmaceuticals, such as erythromycin, ibuprofen, and diclofenac, on the membrane bioreactor has not been tested. The membrane bioreactor uses a heterotrophic biomass, which may be impacted by the presence of pharmaceuticals. Therefore, the adaptive capacity of this biomass was investigated.

The Investigation:

The team used two different concentrations of each compound to investigate the heterotrophic kinetics and organic matter removal efficiency. Over time they could then evaluate any changes to the biomass. Both the low concentration and the high concentration trials inhibited the biomass in one month or less. The system was unable to maintain its normal level of function after the introduction of pharmaceuticals.

Future Needs:

Further work needs to be done to develop a more robust biomass. There are many other organic contaminants of concern in addition to pharmaceuticals, such as those found in personal care products and industrial runoff. As modern life creates more kinds of contamination in our waste streams, new treatments to maintain clean water must be created.

Bermudez L, Sanchez E, Diaz J, et al. Impacts of organic emerging contaminants (erythromycin, ibuprofen, and diclofenac) on the performance of a membrane bioreactor treating urban wastewater: a heterotrophic kinetic investigation. Membranes (Basel). 2023 Jul 27;13(8):697. doi: 10.3390/membranes13080697. PMID: 37623758

 

Shikonin is a naturally occurring compound found in Gromwell roots, traditionally used in Chinese medicine for various purposes. Interestingly, shikonin and the related naphthoquinone compounds are responsible for the red/purple color of the roots. Studies have shown that shikonin itself has antibacterial, anti-inflammatory, and anti-tumor effects. Recently, a new study investigated the effects on brain tumors.

The difficulty in treating brain tumors is the presence of the blood-brain barrier and also the blood-tumor barrier. These barriers prevent most small molecules from crossing, and therefore brain tumors are protected from most chemical treatments. Furthermore, the current standard treatment for glioblastoma includes chemotherapy with temozolomide, however, the glioblastoma cells very quickly develop resistance to this chemical.

In vitro studies showed that shikonin does inhibit glioblastoma cells quite effectively. Therfore, an alternative method of delivery was attempted to cross the barriers. First, lab mice were infused with cancer cells directly into the thalamus. Next, direct intracranial infusion of shikonin was applied into the same spot. By this method, the blood-brain and blood-tumor barriers are physically passed through by the injection needle. In this way, the effect of the treatment in a living system can be observed.

For this study, 2 different brain cancer cell lines were used. The mice from either group, when treated with shikonin, showed significantly longer survival times. Some of the mice were treated with combination of shikonin and temozolomide or temozolomide alone. The effects of the combination treatments were inconclusive. Once cancer cell line seemed to have even better success with a combination treatment, but the other line did not. In addition, neither cancer line, when treated with temozolomide alone, showed significant improvement.

Although shikonin has poor bioavailability and cannot cross the blood-brain barrier on its own, most chemical treatments will face the same problem. Therefore, using direct intracranial infusion may help develop more effective treatment for brain tumors.

Maeoka, R, Ouji Y, Nakazawa T, et al. Local administration of shikonin improved the overall survival in orthotopic murine glioblastoma models with temozolomide resistance. Biomed Pharmacother. 2023 Oct:166:115296. PMID: 37557011

 

Oridonin is a naturally occurring chemical that can be extracted from the Chinese sage bush Isodon rubescens, formerly known as Rabdosia rubescens. It is used in traditional Chinese medicine to block cancer metastasis and recurrence and to overcome therapeutic resistance. Recently, a new study has been completed which looked at its effects on a lung cancer xenograft mouse model.

To begin, the mice were injected subcutaneously with human lung cancer cells. The tumors were allowed to grow and develop to a specified volume prior to beginning treatment. Once the tumors were developed, oridonin was injected intraperitoneally five times each week. In addition, NK-92MI cells were injected intravenously once a week. These “natural killer cells” are a naturally occurring part of an immune system. However, the mouse model used for this experiment does not have a functioning immune system. Therefore, the killer cells were injected during the study.

The results of the study showed that oridonin treatment enhanced the activity of the natural killer cells against all three different cancer cells lines studied. It was also revealed that ordidonin seems to be non-toxic to the liver and kidney. This is exciting because selectively harming only cancer cells without effecting normal cells always proves to be a challenge.

Understanding of the immunostimulatory effect of oridonin on NK cells more thoroughly may help to develop a new lung cancer treatment.

Hwang T, Chang C. Oridonin enhances cytotoxic activity of natural killer cells against lung cancer. Int Immunopharmacol. 2023 Sep;122:110669. doi: 10.1016/j.intimp.2023.110669. PMID: 37480753

Prostate cancer is the second most common cancer worldwide, leading to more than one million diagnoses per year. Metastatic prostate cancer is commonly treated with hormone therapy. These cancer cells need activation of the androgen receptor to divide, so drugs that drop testosterone levels block tumor growth. Unfortunately, resistance to hormone therapy tends to develop in one to two years.

Resistance often involves cancer cells reactivating the androgen receptor without needing testosterone. The authors of a recent study were interested in finding a way to re-inactivate it. The protein CDK7 is an attractive drug target because it is involved in both androgen receptor regulation and cell cycle regulation. The drug CT7001 inhibits CDK7, and shows promising experimental results in several other cancers. Therefore, these authors tested the effectiveness of CT7001 in prostate cancer.

They found that in prostate cancer cell lines, CT7001 binds to CDK7. This molecule also inhibits the androgen receptor, inhibits cell growth, and causes cell cycle arrest. The authors then treated mice carrying prostate cancer tumors with CT7001, or enzalutamide (a drug that blocks testosterone), or both. CT7001 shrank tumors, and the combination of CT7001 and enzalutamide worked even better.

The in vitro and mouse evidence from this study suggests that this chemical could help fight this disease, especially in combination with hormone therapy drugs.

Constantin T, Varela-Carver A, Greenland K, et al. The CDK7 inhibitor CT7001 (samuraciclib) targets proliferation pathways to inhibit advanced prostate cancer. Br J Cancer. 2023 Jun;128(12):2326-2337. PMID: 37076563. doi: 10.1038/s41416-023-02252-8

 

Okadaic Acid is an aquatic toxin that may be accumulated in the fatty tissues of shellfish and other fish. It is produced by microalgae, particularly troublesome during algal blooms, and also leads to contamination of the water itself. This compound is stable even when heated to high temperatures, so boiling or cooking contaminated water or food sources will not destroy the toxin. Consumption of these materials poses a risk to human health causing a variety of severe health problems.

Because there is no easy treatment for contaminated materials, many countries have set strict regulations for acceptable levels of this toxin in seafood. However, the traditional testing methods require highly complex labor-intensive and time-consuming sample preparation. In order to efficiently screen a large number of samples, a new method is necessary.

A New Testing Method

Therefore, a new polarization fluorescence immunoassay (FPIA) has been developed. This FPIA method uses a portable analyzer, requires only minimal sample preparation, and each test requires only 5-10 minutes to complete. First, a fluorescent tracer molecule selectively binds with okadaic acid. Then, this conjugate molecule interacts with specific anti-okadaic acid monoclonal antibodies. Several reference samples with known concentrations are analyzed, and then the actual sample is analyzed and compared.

The Traditional Testing Methods

Previous methods relied on either ELISA (enzyme-linked immunosorbent assay), requiring several hours and tedious sample preparation, or ICA (immunochromatographic analysis), requiring 10-40 minutes and more limited level of detection. In addition, these methods require the sample testing to be performed in a laboratory, which is less convenient.

Both climate change and pollution are leading to more frequent algal blooms in recent decades. Therefore, the ability to monitor for these toxins is becoming increasingly important.

Several of these reference compounds are available at LKT Labs including: Okadaic Acid, Okadaic Acid Ammonium, and Okadaic Acid Sodium.

Hendrickson O, Mukhametova L, Zvereva E, et al. A sensitive fluorescence polarization immunoassay for the rapid detection of okadaic acid in environmental waters. Biosensors (Basel). 2023 Apr 16;13(4):477. doi: 10.3390/bios13040477. PMID: 37185552