Mark Cunningham

β-cembrenediol ((1S,2E,4R,6R,-7E,11E)-2,7,11-cembratriene-4,6-diol) is a diterpenoid that is isolated from the leaves of nicotiana tabacum (tobacco) plants. β-cembrenediol has been found to have neuro-protective effects in mice, reducing ischemic brain injury. The effect was found in both pre-treated and post-injury mice. It does this by increasing the phosphorylation of Akt, a well-known neuroprotective pathway. Additionally, β-cembrenediol and analogs have been found as anti-migratory compounds in wound healing assays with prostate cancer cells.

β-cembrenediol, and it’s C-4 epimer α-cembrenediol are in stock and available for research use.

C1649 β-cembrenediol

C1648 α-cembrenediol

C1650 β-cembrenediol Methyl Ether

Martins A. H. , Hu, J., et al. “Neuroprotective activity of (1S,2E,4R,6R,-7E,11E)-2,7,11-cembratriene-4,6-diol (4R) in vitro and In vivo in Rodent Models of Brain Ischemia” Neuroscience. 2015, Apr 16; 291: 250-259. Doi: 10.1016/j.neuroscience.2015.02.001

Baraka, H. N., Khanfar, M. A., et al. “Bioactive Natural, Biocatalytic, and Semisynthetic Tobacco Cembranoids” Planta Med 2011 Mar:77(5):467-476. Doi: 10.1055/s-0030-1250478.

LKT Laboratories provides custom analytical services for your naturally sourced products. These services include extraction, isolation, identification, quantitation, contamination testing and more. Our fully equipped lab includes UHPLC and LC/MS with ESI and APCI capabilities allowing for trace level detection. Our state-of-the-art analysis can can help solve a variety of your analytical and testing needs:

Quality control testing

Batch-to-batch variations

Stability testing in regular or accelerated storage conditions

Comparison testing of natural products with respect to different parts of the plant, such as seeds, leaves, bark, and roots, or with respect to different seasonal changes natural products undergo

Impurity testing

Product verification

Our friendly and responsive chemists are flexible to your needs and provide you with detailed reports and full documentation on the analysis.

Additionally, LKT also carries a variety of popular natural product standard kits to be used with your own analytic instruments. Besides standard samples, each kit also includes standard sample preparation instructions and liquid chromatogram testing conditions, so they can be easily used to screen your samples. Don’t have an HPLC? No problem! Contact us and we can do the analysis for you using your natural product samples.

AS112 Grape Skin Standards Kit

AS114 Citrus Flavonoids Standards Kit

AS111 Berry Flavonoids Standards Kit

AS109 Soy Isoflavone Standards Kit

AS107 Caffeic Acid Esters Standards Kit

AS104 Camptithecins Standards Kit

LKT Laboratories is pleased to introduce new options for those researchers working with the organosulfur compounds R,S-Sulforaphane and R-Sulforaphane. Both products are now offered at two different purity specifications: ≥99% and ≥97%. Investigators will have the freedom to pick the product that suits their research needs and budgetary constraints.

Sulforaphane is a molecule of significant importance to the scientific community. Since it was first studied in the early 90’s by Talalay, et al. at Johns Hopkins as a major inducer of anticarcinogenic, phase II enzymes, sulforaphane has seen a steady rise in research focus and publications. It has been a part of thousands of major studies, including a record 185 published works in 2016.

LKT Laboratories was the first company to make this compound commercially available in 1995, and continues to be its leading supplier. LKT’s purity standard over the past two decades has always been ≥98%, but with increasing demand for both ultra-pure material and flexibility for researchers, LKT is raising the purity specification on standard product to ≥99% (without increasing its price) as well as introducing the ≥97% version at reduced cost.

The new high purity ≥99% R,S-Sulforaphane and R-Sulforaphane are the purest materials commercially available. They are perfect for long-term projects that may involve animals or other prolonged application periods. Sulforaphane has strict storage requirements to preserve its stability and degrades much more quickly at lower starting purities, which is why LKT recommends the high purity version for projects such as these.

For those looking to use the compound in a shorter time frame, R,S-Sulforaphane and R-Sulforaphane may now be purchased at a ≥97% purity specification at a significantly reduced cost. For research with an immediate application, this product may be a more accessible option.

R,S-Sulforaphane is a synthetic organosulfur compound that displays a variety of interesting characteristics, including anticancer, antimicrobial, antioxidative, anti-inflammatory, and neuroprotective activities. It is a phase II enzyme inducer and induces the expression of the transcription factor Nrf2.

R-Sulforaphane is found naturally in cruciferous vegetables such as broccoli, and has been continuously studied for its health benefits, sometimes showing increased effectiveness compared to its synthetic R,S counterpart in various models.

S8044 R,S-Sulforaphane, High Purity (≥99%)

S8047 R,S-Sulforaphane, Research Grade (≥97%)

S8046 R-Sulforaphane, High Purity (≥99%)

S8048 R-Sulforaphane, Research Grade (≥97%)

1. Zhang Y, Talalay P, Cho CG, Posner GH (March 1992). “A major inducer of anticarcinogenic protective enzymes from broccoli: isolation and elucidation of structure“. Proc. Natl. Acad. Sci. U.S.A. 89 (6): 2399–2403.

Heat shock protein 90 (HSP90) is a molecular chaperone that plays a key role in protein folding and stabilization. Many HSP90 client proteins are overexpressed in cancer cells, and the cancer cells are dependent on HSP90 for growth and survival. Therefore, HSP90 has become a popular therapeutic target for many cancers.

In a recent study it was demonstrated that geldanamycin enhances retrograde transport of the bacterial protein toxin Shiga toxin to the Golgi apparatus in human epidermoid laryngeal carcinoma cells. This activity was shown to be partially mediated by activation of the p38-MK2 pathway, rather than a direct interaction with HSP90.

Although inhibition of HSP90 appears to be an effective target against cancer, geldanamycin is limited by its low aqueous solubility and high toxicity. Consequently, geldanamycin derivatives with improved pharmacological profiles have been developed. These include 17-Allylgeldanamycin and 17-Dimethylaminoethylamino demethoxygeldanamycin.

There are several interesting HSP90 inhibitors available through LKT Labs:

G1646 Geldanamycin

A0025 17-Allylaminogeldanamycin

D4802 17-Dimethylaminoethylamino demethoxygeldanamycin

A5313 Andrographolide

H1669 Herbimycin A

L8377 Luteolin

N5986 Novobiocin Sodium

W3576 Withaferin A

Dyve Lingelem AB, Hjelseth IA, et al. Geldanamycin enhances retrograde transport of Shiga toxin in HEp-2 cells. PLoS One. 2015 May 27;10(5):e0129214. PMID: 26017782.

LKT Labs is happy to announce its annual AACR iPad drawing winner for 2017 – Dr. Haiyan Xiao of Augusta University in Augusta, Georgia.

Dr. Xiao’s primary research focus is based on developing novel and effective vaccines to prevent or treat cancers. Her doctoral dissertation topic was, “Preventive and Therapeutic Roles of Lentiviral Vector Prime and Vaccinia Virus Boost in Mouse Melanoma.” Her dissertation research was published in several well-received articles in the Journal of Immunology. Dr. Xiao’s postdoctoral research is focused on examining how circulating leukocytes interact with the vascular endothelium during chronic inflammatory conditions such as cancer and sickle cell disease.

Dr. Xiao’s research goals are to study epigenetic changes in the immune system that may contribute to chronic conditions including obesity, diabetes, and cancer. By identifying changes, she hopes to develop new insights into the immunological impact of these chronic conditions. Ultimately, she hopes her research leads to the development of methods for preventing immunological complications associated with chronic conditions.

Researchers from past years hail from a variety of institutions, including:

2016 – Boston University; Boston, MA

2015 – The Wistar Institute; Philadelphia, PA

2014 – Vanderbilt University; Nashville, TN

Add your organization to the list of winners in 2018 when LKT Labs holds its next drawing at the AACR conference in Chicago, IL!

MYC is a frequently deregulated oncogene in many human cancers. It is normally responsible for facilitating cell growth and proliferation through the encoding of transcription factors. Since many types of cancers are dependent on the upregulation of MYC, reducing MYC protein levels by targeting its upstream regulators such as histone deacetylases (HDAC) and phophoinositide 3-kinases (PI3K) may be a viable and effective method of treatment.

CUDC-907 is an inhibitor of PI3K and class I and II HDACs. In a recent study published in Molecular Cancer Therapeutics, it was shown that PI3K and HDAC inhibition synergistically downregulated MYC protein levels and induced apoptosis in “double-hit” (DH) diffuse large B-cell lymphoma (DLBCL) cells. CUDC-907 suppressed the growth and survival of MYC-altered or MYC-dependent cancer cells through this downregulation of the MYC protein. Further research and development utilizing CUDC-907 in MYC-driven cancers may be pursued thanks to this study.

LKT Laboratories carries CUDC-907 as well as a number of other PI3K inhibitors for research use.

C8112 CUDC-907

PI3K inhibitor list

Kaiming S., Ruzanna A., et al. Dual HDAC and PI3K inhibitor CUDC-907 Downregulates MYC and Suppresses Growth of MYC-dependent Cancers. Mol Cancer Ther 2016.

Cabozantinib is a chemotherapeutic compound that has shown potential as a treatment for medullary thyroid cancer and other types of solid tumors. It is primarily known for its inhibition of c-Met and vascular endothelial growth factor receptor 2 (VEGFR-2). By inhibiting these tyrosine kinases Cabozantinib reduces tumor growth, metastasis, and angiogenesis.

In a 2016 study, cabozantinib showed activity against gastrointestinal stromal tumors (GIST). In many cases of GIST, oncogenic signaling is driven by KIT mutations. Tyrosine kinase inhibitors (TKI) such as imatinib have been used to treat GIST, but resistance to TKI caused by secondary mutations to KIT sometimes renders this treatment ineffective.

Cabozantinib was tested in patient-derived xenograft models of GIST, with each model carrying different KIT mutations. Three different models were examined in total, and in all three cabozantinib inhibited proliferative activity, even in those models that were imatinib-resistant. Compared to an imatinib treated group and a control group, cabozantinib also significantly reduced microvessel density in all three models.

From the study it was concluded that cabozantinib shows promising antitumor activity in both imatinib-sensitive and imatinib-resistant GIST models. The activity is achieved through the inhibition of tumor growth, proliferation, and angiogenesis, and may provide a valid alternate treatment method for GIST going forward.

C0006 Cabozantinib

I4802 Imatinib Mesylate

Gebreyohannes Y., Schoffski P., et al. Cabozantinib is Active against Human Gastrointestinal Stromal Tumor Xenografts Carrying Different KIT Mutations. Mol Cancer Ther; 15(12); 2845-52.

Piperlongumine is found in several species of the Piper plant and displays many beneficial characteristics, including antithrombotic, anti-inflammatory, anti-atherosclerotic, and chemotherapeutic activities. Isolated from the fruit of the long pepper, recent research has focused on the ability of this natural product to suppress tumor growth alone and in combination with other chemotherapeutic compounds.

In a study published in 2015, the effects of Piperlongumine were examined in pancreatic cancer cells. Pancreatic cancer is an aggressive malignancy with a relatively poor prognosis, even in cases where it is diagnosed early. There are few good options for chemotherapy.

In vitro, piperlongumine showed inhibition of pancreatic cancer cell lines, inhibited the activation of NF-kB, and also potentiated the apoptotic effects of gemcitabine, a nucleoside analog commonly used in the chemotherapeutic treatment of some cancers. Piperlongumine went on to show significant activity in vivo, where it suppressed tumor growth alone and enhanced the efficacy of gemcitabine in xenograft mouse models. These results were consistent with the downregulation of NF-kB activity and its target genes, decreased proliferation, and increased apoptosis in tumor remnants seen in the model.

The results of the study suggest both potential for piperlongumine as a stand-alone treatment for human pancreatic cancer, as well as a partner-chemotherapeutic with compounds like gemcitabine. Both piperlongumine and gemcitabine are available from LKT Labs for research use.

P3561 Piperlongumine

G1745 Gemcitabine Hydrochloride

Wang Y., Wu X., et al. Piperlongumine Suppresses Growth and Sensitizes Pancreatic Tumors to Gemcitabine in a Xenograft Mouse Model by Modulating the NF-kappa B Pathway. Cancer Prev. Res. Mar;9(3):234-44.

As of October 12th, 2016, LKT Laboratories has earned its MBE certification from the North Central Minority Supplier Development Council. The National Minority Supplier Development Council (NMSDC) advances business opportunities for certified minority business enterprises and is one of the nation’s leading corporate membership organizations.

End users may secure funding for resources provided by trusted minority-owned businesses such as those certified through the NMSDC. LKT Labs is proud to join this network of organizations.

Barasertib, also known as AZD-1152, is a highly selective aurora kinase B inhibitor. As an aurora kinase inhibitor, it has shown the ability to disrupt the cell cycle and consequently induce apoptosis in vitro. It has inhibited the growth of human colon, lung, and haematologic tumor xenografts in immunodeficient mice, and continues to be evaluated in various disease models.

In a 2016 study, barasertib inhibited the growth of small-cell lung cancer cells in vitro and in vivo. Small-cell lung cancer cells are good targets for aurora kinase inhibition, thanks to their rapid proliferation and high rates of MYC family amplification. Inhibitors of mitosis such as aurora kinase inhibitors have been effective in causing apoptosis in cells expressing high levels of MYC in other tumor types.

A panel of small-cell lung cancer lines with and without MYC family gene amplification was screened for growth inhibition by barasertib. The growth inhibition caused by barasertib correlated with cMYC amplification and cMYC gene expression in the panel. Small-cell lung cancer tumors with high cMYC amplification or gene expression may thus be good targets for barasertib and other aurora kinase B inhibitors.

LKT Labs sells a number of aurora kinase inhibitors, including AZD1152:

A9714 AZD-1152-HQPA

M4652 MLN-8237

T5996 Tozasertib

Z4900 ZM-447439 Trihydrate

References:

Helfrich B., Kim J., et al. Barasertib (AZD1152), a Small Molecule Aurora B Inhibitor, Inhibits the Growth of SCLS Cell Lines In Vitro and In Vivo. Mol Cancer Ther; 15(10); 2314-22. PMID: 27496133.